Search results for "Microsatellite Analysis"

showing 3 items of 3 documents

Multilocus microsatellite analysis of European and African Candida glabrata isolates

2016

This study aimed to elucidate the genetic relatedness and epidemiology of 127 clinical and environmental Candida glabrata isolates from Europe and Africa using multilocus microsatellite analysis. Each isolate was first identified using phenotypic and molecular methods and subsequently, six unlinked microsatellite loci were analyzed using automated fluorescent genotyping. Genetic relationships were estimated using the minimum-spanning tree (MStree) method. Microsatellite analyses revealed the existence of 47 different genotypes. The fungal population showed an irregular distribution owing to the over-representation of genetically different infectious haplotypes. The most common genotype was …

0301 basic medicineSettore MED/07 - Microbiologia E Microbiologia ClinicaClonal complexEpidemiologyMultilocus microsatellite analysisCandida glabrataMolecular phylogenyGene locusCentral typeRelated genotypeGenotypeEnvironmental MicrobiologyHaplotypeDNA FungalPriority journalGeneticsAlleleCandidiasisGeneral MedicineClassificationEuropePhenotypeInfectious DiseasesCandida Glabrata; Adhesins; FluconazoleCandidiasiMicrosatelliteMicrosatellite RepeatMicrobiological examinationHumanMicrobiology (medical)GenotypeSettore MED/17 - Malattie InfettiveMicrosatellite DNA030106 microbiologyBiologyEuropeanMicrobiologyArticle03 medical and health sciencesGenetic variationMicrosatellite repeatsGeneticsHumansAlleleGenotypingAllelesScience & TechnologyCandida glabrataMicrosatellite markerHaplotypeAfricanGenetic Variationbiology.organism_classificationNonhuman030104 developmental biologyFungal DNAHaplotypesIsolation and purificationGenetic LociAfricaMultilocus sequence typingFungus isolationGenetic variabilityMicrosatellite genotypeMultilocus Sequence Typing
researchProduct

Potential association of specific Candida parapsilosis genotypes, bloodstream infections and colonization of health workers' hands.

2014

AbstractFungal nosocomial infections continue to be a serious problem among hospitalized patients, decreasing quality of life and adding millions of euros to healthcare costs. The aim of this study was to describe the pattern of fungi associated with the hands of healthcare workers and to genotype Candida parapsilosis isolates in order to understand whether their high clinical prevalence stems from endemic nosocomial genotypes or from the real emergence of epidemiologically-unrelated strains. Approximately 39% (50/129) of healthcare workers were positive for yeasts and among 77 different fungal isolates recovered, C. parapsilosis was the most frequent (44/77; 57%). Twenty-seven diverse geno…

Microbiology (medical)Candida parapsilosismicrosatellite genotypingSettore MED/17 - Malattie InfettiveGenotypeSepsiHospitalized patientsHealth PersonnelFungal pathogenNosocomial fungal infectionInfectious DiseaseBiologyCandida parapsilosisPersistence (computer science)MicrobiologyCandida parapsilosis; Candida species; Candidaemia; fungal pathogens; healthcare workers; microsatellite genotyping; nosocomial fungal infectionsCandidaemiaRetrospective Studienosocomial fungal infectionsSepsisGenotypeCandida speciesDisease Transmission InfectiousHumansColonizationDNA FungalMycological Typing TechniquesCandidaRetrospective StudiesGenetic diversityCross InfectionMolecular Epidemiologyhealthcare workersMedicine (all)Candida parapsilosis; Candida species; Candidaemia; Fungal pathogens; Healthcare workers; Microsatellite genotyping; Nosocomial fungal infections; Candida; Cross Infection; DNA Fungal; Disease Transmission Infectious; Genotype; Hand; Humans; Molecular Epidemiology; Molecular Typing; Mycological Typing Techniques; Retrospective Studies; Sepsis; Health Personnel; Microbiology (medical); Infectious Diseases; Medicine (all)General Medicinebiology.organism_classificationHandMolecular TypingInfectious DiseasesDisease Transmission InfectiouMicrosatellite AnalysisCandida specieHealthcare workerCandida parapsilosiMycological Typing Techniquefungal pathogensHumanClinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
researchProduct

An improved high yield method to obtain microsatellite genotypes from red deer antlers up to 200 years old.

2013

Analysis of DNA from older samples, such as museum specimen, is a promising approach to studying genetics of populations and ecological processes across several generations. Here, we present a method for extracting high quality nuclear DNA for microsatellite analysis from antlers of red deer (Cervus elaphus). The genotyping of individuals was based on nine microsatellite loci. Because the amount of DNA found in antlers was high, we could reduce the amount of sample and chemicals used and shorten the decalcification time in comparison to other methods. Using these methods, we obtained genotypes from antlers up to 200 years old.

animal structuresGenotypeZoologyAntlersBiologyPolymerase Chain ReactionChemistry Techniques Analyticallaw.inventionlawGermanyGenotypeGeneticsAnimalsGenotypingEcology Evolution Behavior and SystematicsPolymerase chain reactionGeneticsDeerMuseumsDecalcification TechniqueNuclear DNAMicrosatellite AnalysisMicrosatelliteCervus elaphusBiotechnologyMicrosatellite RepeatsMolecular ecology resources
researchProduct